Anti-CRISPR-Cas9 antibody [7A9-3A3] ab191468 from Abcam

Product Specs
ItemAnti-CRISPR-Cas9 antibody [7A9-3A3]
CompanyAbcam
Price Pricing Info Supplier Page View Supplier Product Page
Catalog Numberab191468
Quantity10 UG, 100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot, Immunocytochemistry/ Immunofluorescence, Western blot
Conjugate/TagUnconjugated
HostMouse, Nude mouse
Antibody TypePrimary
ClonalityMonoclonal
Clone7A9-3A3
Gene Namecas9
ReactivityStreptococcus pyogenes
TargetCRISPR-Cas9

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Inhibition of lysosome-tethered Ragulator-Rag-3D complex restricts the replication of Enterovirus 71 and Coxsackie A16Journal of Cell BiologyDecember 4, 2023Xinhui Wang, Zhilin Hu, Wei Zhang, Shuwei Wu, Yongjin Hao, Xia Xiao, Jingjing Li, Xiaoliang Yu, Chengkui Yang, Jingfeng Wang, et al.Enterovirus 71 3D (Cat #GTX630193, RRID:AB_2888196; GeneTex), GSDME (Cat #ab215191; Abcam), Cas9 (Cat #ab191468; Abcam), Caspase-3 (Cat #9662s; Cell Signaling Technology), Cleaved-Caspase-3 (Cat #9661; Cell Signaling Technology

(2) Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human SkinACS NanoNovember 14, 2023Juliana Bolsoni, Danny Liu, Fatemeh Mohabatpour, Ronja Ebner, Gaurav Sadhnani, Belal Tafech, Jerry Leung, Selina Shanta, Kevin An, Tessa Morin, et al.purchased from Fisher Scientific (Mississauga, ON, Canada). Mouse monoclonal antibody against Cas9 (ab191468) and live and dead cell assays were purchased from Abcam (Cambridge, MA, USA). ApoE4 (Apolipoprotein

(3) CRISPR/dCAS9-mediated DNA demethylation screen identifies functional epigenetic determinants of colorectal cancerClinical EpigeneticsAugust 24, 2023Juan Ramón Tejedor, Alfonso Peñarroya, Javier Gancedo-Verdejo, Pablo Santamarina-Ojeda, Raúl F. Pérez, Sara López-Tamargo, Ana Díez-Borge, Juan J. Alba-Linares, Nerea González-del-Rey, Rocío G. Urdinguio, et al.using the following primary antibodies (1:1000 dilution in TBS-T with 5% milk): anti-Cas9 (Abcam, #ab191468) and anti- α Tubulin (Abcam, #ab7291). The secondary antibody was a rabbit anti-mouse IgG H&L (Abcam,

(4) Lipopolysaccharide transport regulates bacterial sensitivity to a cell wall-degrading intermicrobial toxinPLOS PathogensJune 26, 2023Kristine L. Trotta, Beth M. Hayes, Johannes P. Schneider, Jing Wang, Horia Todor, Patrick Rockefeller Grimes, Ziyi Zhao, William L. Hatleberg, Melanie R. Silvis, Rachel Kim, et al.hour at RT with shaking in 3% milk+TBST. Primary antibody was applied: 1:1000 mouse anti-Cas9 (Abcam ab191468) in TBST, overnight, at 4C with shaking. Blots were washed four times in TBST. Secondary antibody was

(5) Rapid and Reliable Quantification of Prime Editing Targeting Within the Porcine ABCA4 Gene Using a Bioluminescence Resonance Energy Transfer-Based SensorNucleic Acid TherapeuticsJune 1, 2023Tobias Wimmer, Hannah Sawinski, Anne M. Urban, Jan Motlik, Knut Stiegerof prime editors in HEK293-T cells with an antibody raised against SpCas9 (mouse anti-CRISPR-Cas9; ab191468; Abcam, Cambridge, United Kingdom). HEK293-T cells transfected with PEs were lysed as described above.

(6) Lipopolysaccharide integrity primes bacterial sensitivity to a cell wall-degrading intermicrobial toxinbioRxivMay 6, 2023Kristine L Trotta, Beth M Hayes, Johannes P Schneider, Jing Wang, Horia Todor, Patrick Rockefeller Rockefeller Grimes, Ziyi Zhao, William L Hatleberg, Melanie R Silvis, Rachel Kim, et al.hour at RT with shaking in 3% milk+TBST. Primary antibody was applied: 1:1000 mouse anti-Cas9 (Abcam ab191468) in TBST, overnight, at 4C with shaking. Blots were washed four times in TBST. Secondary antibody was

(7) Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinomaClinical epigeneticsApril 29, 2023Sgro A, Cursons J, Waryah C, Woodward EA, Foroutan M, Lyu R, Yeoh GCT, Leedman PJ, Blancafort P.polyclonal (Abcam, Cat. No. ab122507, 1:300), and α-SpCas9 mouse monoclonal [7A9-3A3] (Abcam, Cat. No. ab191468, 1:500). The secondary antibodies used were Alexa Fluor 488-conjugated goat α-mouse (Thermo Fisher Scientific,

(8) A molecular glue approach to control the half-life of CRISPR-based technologiesbioRxivMarch 24, 2023Vedagopuram Sreekanth, Max Jan, Kevin T. Zhao, Donghyun Lim, Jessie R. Davis, Marie McConkey, Veronica Kovalcik, Sam Barkal, Benjamin K. Law, James Fife, et al.were subjected to immunoblotting with a monoclonal antibody anti-Cas9 antibody (Abcam: ab189380 or ab191468), anti-FLAG antibody (Cell Signaling Technologies: 2368S), and a monoclonal anti-β-actin antibody (Cell Signaling Technologies

(9) Genome-scale CRISPR screening in a single mouse liverCell GenomDecember 14, 2022Keys HR, Knouse KA.(ThermoFisher Scientific). The following primary antibodies were used: Cas9 (1:200, clone 7A9-3A3, Abcam ab191468), asialoglycoprotein receptor 1 (ASGR1) (1:500, clone 114, Sino Biological 50083-R114), mCherry (1:500,

(10) Non-viral CRISPR activation system targeting VEGF-A and TGF-β1 for enhanced osteogenesis of pre-osteoblasts implanted with dual-crosslinked hydrogelMaterials Today BioDecember 1, 2022Guo Chen, Shaohui Deng, Mingxiang Zuo, Jin Wang, Du Cheng, Bin Chen0.1% Tween 20) for 1 h. Cells were incubated with a 200 × dilution of anti-CRISPR-Cas9 antibody (ab191468, Abcam, Cambridge, UK) in PBST plus 1% BSA in a humidified chamber overnight at 4 °C. Cells were washed

(11) A loss-of-adhesion CRISPR-Cas9 screening platform to identify cell adhesion-regulatory proteins and signaling pathwaysNature CommunicationsDecember 1, 2022Martin F. M. de Rooij, Yvonne J. Thus, Nathalie Swier, Roderick L. Beijersbergen, Steven T. Pals, Marcel Spaargarenlenti-Guide-Puro Brunello kinome library (Addgene #1000000082). Antibodies: mouse anti-Cas9 (Clone 7A9-3A3, #ab191468, Abcam), phycoerythrin-conjungated goat F(ab’)2 anti-mouse IgG1 (Polyclonal, #1072-09, Southern Biotech),

(12) Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editingNature CommunicationsDecember 1, 2022Duško Lainšček, Vida Forstnerič, Veronika Mikolič, Špela Malenšek, Peter Pečan, Mojca Benčina, Matjaž Sever, Helena Podgornik, Roman Jeralaantibodies. Primary antibodies used for western blotting were as follows: mouse anti-CRISPR-Cas9 antibody (ab191468) from Abcam and rabbit anti-Alpha/Beta tubulin antibody (2148) that were purchased from Cell Signaling Technology

(13) Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stagesCell ReportsSeptember 6, 2022Hironori Hojo et al.RRID:AB_731605 Rabbit polyclonal anti-CBFb antibody,Abcam,ab195411 Mouse monoclonal anti-CRISPR-Cas9 antibody,Abcam,ab191468; RRID:AB_2692325 Rabbit polyclonal to Histone H3 antibody,Abcam,ab1791; RRID:AB_302613 Alexa Fluor

(14) CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated FactorsBiomoleculesMay 14, 2022Guiro J, Fagbemi M, Tellier M, Zaborowska J, Barker S, Fournier M, Murphy S.Antibodies,Reference AffiniPure Donkey Anti-Rabbit IgG (H + L),Jackson ImmunoResearch 711-005-152 Anti-CRISPR-Cas9,Abcam ab191468 CDK12,LS Bio LS-C288466-100 CDK12,Novus Biologicals NB100-87011 Cyclin K,Bethyl Laboratories A301-939A Oct-1,Bethyl Laboratories

(15) Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9Nature CommunicationsDecember 1, 2021Daniel M. Sapozhnikov, Moshe SzyfTBST and protein was detected with either mouse Anti-CRISPR-Cas9 primary antibody [7A9-3A3] (Abcam, ab191468) (1/2000 dilution) or monoclonal Anti-β-Actin primary antibody produced in mouse (Sigma, A2228) (1/5000

(16) Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector deliveryNucleic Acids ResearchNovember 18, 2021Qian Wang, Jin Liu, Josephine M Janssen, Francesca Tasca, Hailiang Mei, Manuel A F V Gonçalvesincubated overnight at 4°C with primary antibodies raised against S. pyogenes Cas9 (Abcam; Cat. No.: ab191468; 1:1000 dilution), myosin heavy chain (Sigma-Aldrich; Cat. No.: M4276; 1:500 dilution), sarcomeric α-actinin

(17) Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complexScience advancesOctober 8, 2021Ban Y, Yu T, Feng B, Lorenz C, Wang X, Baker C, Zou Y.anti-synaptic vesicle 2 (SV2) (Developmental Studies Hybridoma Bank), and anti–CRISPR-Cas9 (Abcam, ab191468). Hippocampal neuron and HEK293T coculture The mixed culture assay was performed as described with

(18) Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3Cell stem cellSeptember 2, 2021Padmanabhan Nair V, Liu H, Ciceri G, Jungverdorben J, Frishman G, Tchieu J, Cederquist GY, Rothenaigner I, Schorpp K, Klepper L, et al.or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Mouse monoclonal Anti-CAS9,Abcam,"ab191468, RRID:AB_2692325" Mouse monoclonal Anti-GAPDH,EMD Millipore Corp,"CB1001–500UG, RRID:AB_2107426" Mouse

(19) Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras(G12D) in pancreatic cancerLife science allianceSeptember 1, 2021McAndrews KM, Xiao F, Chronopoulos A, LeBleu VS, Kugeratski FG, Kalluri R.9102" Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204),Rabbit,"1:1,000","CST, 4376" Cas9,Mouse,1:500,"Abcam, ab191468" HRP-conjugated β-actin,-,"1:25,000","Sigma-Aldrich, A3854" Anti-rabbit HRP-conjugated,-,"1:5,000","CST,

(20) Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathwayCellAugust 19, 2021Evavold CL, Hafner-Bratkovič I, Devant P, D'Andrea JM, Ngwa EM, Boršić E, Doench JG, LaFleur MW, Sharpe AH, Thiagarajah JR, et al.from Takara (631131, 1:1000), spCas9 from Cell Signaling Technologies (65832S, 1:1000) and from Abcam (ab191468, 1:1000), RagA from Cell Signaling Technologies (4357S, 1:1000), RagC from Cell Signaling Technologies

Be the First to Write a Review!

Anti-CRISPR-Cas9 antibody [7A9-3A3] from Abcam

Abcam

Citations (57)

Reviews

Anti-CRISPR-Cas9 antibody [7A9-3A3] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map